Based on the degradation studies submitted during the first EU review of glyphosate (2001), it was concluded that glyphosate was degraded in soil over time by micro-organisms. The principal soil metabolite was aminomethylphosphonic acid (AMPA). The maximum amount of AMPA detected ranged from 15 to 29% of the total glyphosate applied as reported in the glyphosate Monograph. These studies also established that AMPA is further degraded by soil micro-flora, although at a slower rate than glyphosate. They also demonstrated that 41.6% to 79.6% of the applied glyphosate was mineralised to CO2 and 18.9 to 19.8% bound residues were formed. Volatiles other than CO2 remained, when measured, at <0.3% of AR: The results of the new studies and the now considered acceptable study of Kesterson & Atkins (1991, BVL no 1932061)/Honegger (1992, BVL no 2325652) are consistent with the conclusions of the first EU review of glyphosate (2001) and confirm that glyphosate is degraded in soil over time by micro-organisms in the laboratory under aerobic conditions. It is also confirmed that the only significant soil metabolite of glyphosate is AMPA. However, the maximum amount of AMPA detected from all studies ranged from 13.3 to 50.1% AR. The maximum occurrence of 50.1% is recommended to be used in further risk assessment. The studies demonstrate that at 20 and 25°C, 16.9 to 79.6% AR was mineralised to CO2 and 2.5 to 43.2% AR bound residues were formed. At 10°C, 48.2% AR CO2 and 2% AR bound residues were formed. Several other minor components were also detected in the new aerobic soil metabolism study; however, no single metabolites was detected in an amount greater than 2.3% of the applied glyphosate. Volatiles other than CO2 were not detected.