The rate of degradation in soil of acibenzolar-S-methyl was evaluated during the original EU review. Additional data were not required as a result of the review, however to overcome some deficiencies (lack of replicates, soil < 7 and unidentified peak) of the initial degradation studies, an additional laboratory soil degradation study has been performed (Miner, 2102). The new study confirmed the main route of degradation of acibenzolar-S-methyl via hydrolysis to the transient acid metabolite CGA210007, formed at levels up to 95% applied radioactivity. Subsequent hydroxylation of CGA210007 in this study led to the observation of a new soil metabolite, SYN546642, which was observed at levels up to 39% applied radioactivity. The fate of acibenzolar-S-methyl under strict anaerobic conditions is unknown while the degradation of CGA210007 was shown null. However, such condition is not likely to occur and last for the intended use (during the Spring and Summer months when the compound is applied). In conclusion, only two major metabolites of acibenzolar-S-methyl, CGA210007 and SYN546642, are observed under aerobic soil conditions. The formation and degradation of non-extractable (bound) residues and mineralisation to carbon dioxide (19-23% AR for NER and 46-53. for CO2 at 125 DAT) represent the main overall pathway for the metabolism of acibenzolar-S-methyl.