The rate of degradation of the a.s. was calculated using non linear single first order regression. The DT50 were 329 and 1028 days at 22°C Two metabolites detected by TLC and HPLC analyses were found at low level in both soils: 0.5-0.6% and 0.7- 1.0% at day 120, respectively for M2 and M3. An attempt to identify them using HPLC/MS has not been successful. Mineralization accounts for 4.4-1.3% AR at day 120. Bound residues account for 17.6-10.2% AR at day 120. Further analysis of the bound residues at day 92, 181 and 365 showed that 3.11-13.31%, 3.21-14.46% and 1.27- 3.25% of the applied radioactivity was associated with the humin (irreversibly bound), fulvic acid and humic acid fractions, respectively. Additional study with higher treatment rate  The radio-HPLC analyses of the first soil (Péage de Roussillon) showed the presence of 9 metabolites accounting for 0.86% AR. The following compounds were identified by HPLC-MS : one impurity present in the dosing solution (LS880226), one artefact due to the extraction solvent (LS860976), 2 metabolites (RPA401527, LS860551). Other compounds could not be identified. Through hydrolytic reaction, the hydroxylated compound (LS860551) is formed from one a.s. diastereoisomer (LS850646), to form LS860364 (not identified in this study). This compound is easily oxidized to a possible hydroxyperoxy compound further converted to the lactone (RPA 401527). Some CO2 originating from the phenyl ring was also identified. Study on varying moisture, dose rate and soil viability The degradation of bromuconazole is very limited in this experiment with incubation under aerobic conditions at 20°C. There was no definite trend in breakdown. Minor differences in metabolites pattern were observed between the different incubation conditions. 27 degradates were detected at levels between 0.5% and 5.2% each of the applied dose, up to 20 being found in one system. 7 metabolites (LS-860551, LS-860550, LS-802050, LS-870353/ LS-861590, LS-861019 and LS- 860976) have been confirmed by LC-MS . Minor differences in metabolites pattern were observed between the different incubation conditions. The metabolite M2 reached a maximum level of 4.2% AR at day 100. The metabolite M6 was tentatively assigned as a compound of MWt. 257
The metabolite M8 is confirmed as being LS-802050 (not detected in this experiment)
The metabolite M9 would be formed of two peaks, tentatively identified as LS-870353 and LS-861590
The metabolites M10 and M15 have been tentatively identified as the two diastereoisomers of a monochlorophenyl synthetic impurity.
Metabolites M11 and M13 have been tentatively identified as methylated diastereoisomers of LS-860551 and LS-860550, artifacts due to extraction with methanol.
Metabolites M12 and M14 have been tentatively identified as the two diastereoisomers of a monochlorophenyl synthetic impurity. The mineralization reached a maximum level of 1-2% AR after 100 days under aerobic conditions (normal and reduced dose). Bound residue level was 5-9% AR after 100 days under aerobic conditions.