Linear alkylbenzenesulfonate (LAS) is a major synthetic surfactant, which is commonly used in laundry detergents. The degradation of LAS is important because it is a source of carbon in our environment, and worldwide over 2 million tons of LAS is produced ([http://www.ncbi.nlm.nih.gov/pubmed/19915037|Schleheck et al., 2010]). Commercially, LAS contains a mixture of about twenty different congeners, which are degraded into compounds such as sulfophenylcarboxylates (SPCs) ([http://www.ncbi.nlm.nih.gov/pubmed/15240283|Schleheck et al., 2004]). 3-(4-Sulfophenyl)butyrate is degraded by Comamonas testosteroni KF-1 to 4-sulfoacetophenone and 4-sulfophenol. The initial steps in 3-(4-sulfophenyl)butyrate degradation are analogous to beta-oxidation, with the formation of a CoA ester, dehydrogenation, hydration, and oxidation ([http://www.ncbi.nlm.nih.gov/pubmed/19915037|Schleheck et al., 2010]). The hydrolysis of the ester to acetate and 4-sulfophenol is followed by monoxygenation to 4-sulfocatechol. Ortho ring cleavage, desulfonation, and sulfite oxidation lead to intermediary metabolites.