Cyanuric Acid
Cyanuric acid is widely used in swimming pools and is generated as a metabolic intermediate during the bacterial metabolism of s-triazine pesticides ([http://www.ncbi.nlm.nih.gov/pubmed/10427042|Karnes, 1999]). It is further metabolized by bacteria and fungi via hydrolytic enzymes that ultimately release the nitrogen as ammonia. For twenty-five years, it was thought that cyanuric acid metabolism proceeded through urea as an intermediate. More recent studies ([http://www.ncbi.nlm.nih.gov/pubmed/16085834|Cheng et al., 2005]) have shown that urea is derived from spontaneous decarboxylation of allophanate during purification of metabolites. Thus, cyanuric acid metabolism to 3 mol of ammonia is now shown to proceed exclusively via biuret and allophanate.